Research Assistant III

Animal Physiology Research Assistant

Job-Specific Responsibilities

We are seeking an Animal Physiology Research Assistant (RA) who is enthusiastic and excited about the in vivo physiology work we do in our lab, wants to make a strong scientific contribution to our research, and has the interest in growing within the role. Reporting to the Director of Animal Research, this Research Assistant will work with genetically engineered mouse models and perform a wide variety of physiology experiments, including studies implementing advanced physiological instrumentation and surgeries. It is an opportunity to learn and perform advanced techniques in molecular biology, protein and RNA purification and analysis, mammalian tissue culture, biochemical assays, immunostaining, and microscopy, while supporting various research projects across the lab. The RA will also follow up experiments by organizing, processing, and reporting data. As such, this RA is expected to be fully-engaged in the intellectual activities of the lab and to actively participate in lab meetings and discussions.

Additional duties and responsibilities include, but are not limited to, the following:

  • Performing mouse metabolic physiology experiments

  • Maintaining mouse colonies, including breeding and genotyping

  • Processing mouse blood and tissue samples for protein and RNA analysis

  • Maintaining cell cultures and performing cell culture experiments

  • Assisting various lab members (across various projects) with mouse physiology experiments

  • Performing and supporting the analysis, interpretation, and reporting of data utilizing multiple software programs and scientific instrumentation

  • Participation and intellectual contribution to lab meetings, journal clubs, and data discussions

  • Serving as back-up to the Director as needed

  • Performing other duties as assigned


PLEASE NOTE: This position has a term end date of 12/31/2023, with the possibility of extension based on funding. It is also full-time, benefits-eligible with a 40-hour workweek.

PLEASE NOTE: The primary work location for this position is Boston, MA and in order to be on Harvard payroll, you must reside in one of the following states – MA, NH, RI, ME, CT, VT, NY, or MD. Until our return to campus and full operational status, which is still to be determined, this interview process and position will take place in a slightly modified manner – including virtual interviews and a combination of remote and on-site onboarding and work. Full on-campus/in-person work is expected, though, upon start date – in accordance with approved COVID-related guidelines and protocols. Shifts are also likely to be irregular (potentially including weekends, off-hours, holidays, and on-call back-up coverage) and may therefore require schedule flexibility.

PLEASE NOTE: Finalists will be required to complete an occupational health screening.

Basic Qualifications

  • 3+ years of related work experience required; a combination of education and experience may be considered

  • Experience in basic and advanced molecular and cellular biology techniques required

Additional Qualifications and Skills

The following job-specific skills and competencies are preferred:

  • Bachelor's degree strongly preferred

  • Experience handling small animals in a research setting

  • IOS operating system device familiarity

  • Microsoft Office Suite experience (including Word, Excel and PowerPoint

  • Interested in making a commitment to grow in the position and assume increasing levels of responsibility

  • Proven high level of initiative and motivation, and experience working independently and with a team

  • Proven excellence in communication, organization, time management, and record-keeping skills; demonstrated strong organizational skills

  • Excitement and interest specifically for in vivo physiology work as a profession

The following cultural competencies are also preferred:

  • Awareness of and aptitude to appropriately and effectively understand, respect, and adapt to cultural and identity-based difference within group environments

  • Knowledge of and commitment to concepts and issues tied to social justice, diversity, equity, and inclusion

  • Skills related to creating and supporting an environment that allows for inclusion, effective intercultural engagement, and personal humility and authenticity

Physical Requirements

  • Ability to lift 30 pounds, climb a three-step ladder, and safely handle a mouse cage

  • Ability to and comfortability with handling live mice, conducting mouse surgeries, harvesting tissues, and sacrificing mice


Additional Information

Harvard University offers an outstanding benefits package including:

  • Time Off: 3-4 weeks paid vacation, paid holiday break, 12 paid sick days, 12+ paid holidays, and 3 paid personal days per year.

  • Medical/Dental/Vision: Excellent and affordable medical, dental & vision plans, with coverage beginning as of an employee's start date.

  • Retirement: University-funded retirement plan with full vesting after 3 years of service.

  • Tuition Assistance & Reimbursement Programs: $40 per class at the Harvard Extension School, discounted options through participating Harvard graduate schools, and reimbursement options for accredited schools outside of Harvard.

  • Transportation: 50% discount on MBTA passes, as well as additional options (rideshare, biking, etc.) to assist employees in their daily commute.

  • Wellness & Professional Development: Programs and classes at little or no cost, including stress management, hard & soft skills development, career planning, massages, nutrition, meditation, and complimentary health services. Additionally, access to Harvard athletic facilities, libraries, campus events -- and many discounts for various cultural and leisure activities throughout metro Boston.


Join the Harvard T.H. Chan School of Public Health to support our mission of health research and education, and to be a part of the oldest institution of higher learning in the country!


  • The Harvard T.H. Chan School of Public Health does not provide visa sponsorship, now or in the future, for staff positions.

  • Harvard University requires pre-employment reference checks and background screenings.

  • This position has a 90 day orientation and review period. The O&R period will be waived for any internal Harvard employee transfers.

The University requires all Harvard community members to be fully vaccinated against COVID-19 and remain up to date with COVID-19 vaccine boosters, as detailed in Harvard’s Vaccine & Booster Requirements. Individuals may claim exemption from the vaccine requirement for medical or religious reasons. More information regarding the University’s COVID vaccination requirement, exemptions, and verification of vaccination status may be found at the University’s “COVID-19 Vaccine Information” webpage.


Job Function



USA - MA - Boston

Job Code

403116 Research Assistant III Lab




Molecular Metabolism

Time Status


Salary Grade



55 - Hvd Union Cler & Tech Workers

Appointment End Date


Pre-Employment Screening

Criminal, Identity

Commitment to Equity, Diversity, Inclusion, and Belonging

At the Harvard T.H. Chan School of Public Health, we believe that diversity is integral to the Harvard experience and our mission of improving public health education, research, and policy. Diversity of cultural backgrounds, identities, lived experiences, perspectives, and ways of understanding the world enriches our community and enables us to best meet the public health needs of the United States and the world. Ongoing learning and development related to diversity allows for both individual and institutional growth and is necessary to foster and sustain a culture of inclusion. To achieve this, we are committed to ensuring equitable access to opportunities for learning, living, and working at the Harvard Chan School. We maintain an unwavering dedication to diversity, inclusion, and belonging as core to our institutional values and to actively counter, minimize, and address racism, sexism, xenophobia, homophobia, gender bias, and all forms of discrimination.

Learn more about the School’s mission, objectives, core values, and our diversity, inclusion, and belonging initiatives.

EEO Statement

We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, gender identity, sexual orientation, pregnancy, and pregnancy-related conditions, or any other characteristic protected by law.